IgG 101 (C vs Fc confusion)

A lesson learned: read twice, clone once.

Recently a client asked us to fuse a gene with a human Fc. Without double checking the request we fused the requested gene with the entire human G1 constant region and delivered it to the client. You can only imagine our embarrassment when the client called and told us that we had assembled a wrong construct.

Alex

Omnirat

Hello Friends,

What do you think about the future of antibody humanization? Would we still have need for it two, three, five years from now? Would new humanized animal strains and single-cell technologies make this art obsolete? From where I stand it definitely looks like. If cloning antibodies from potent individuals has or could have some legal and moral consequences, use of humanized animals has not. The recently developed Omnirat is a huge step in this direction. Want to know more - read the original paper in the Journal of Immunology, 190:1481, 2014.

Share your thoughts.

Alex